Title Page
Contents
ABSTRACT 8
Ⅰ. Introduction 9
Ⅱ. Materials and Methods 11
2.1. Sample collection 11
2.2. Viral DNA extraction 11
2.3. TTCaV detection 11
2.4. PCR amplification of entire TTCaV genome 12
2.5. Phylogenetic tree and sequence identity analysis 13
2.6. Similarity plot and recombination analysis 13
Ⅲ. Results 15
3.1. TTCaV Incidence in Dogs 15
3.2. Complete Genomic Analysis of TTCaV 15
3.3. SimPlot and Recombination Analysis of TTCaV 16
Ⅳ. Discussion 24
Ⅴ. Conclusion 26
References 27
Abstract (in Korean) 31
Table 1. Primer sets used for the detection of partial and full TTCaV genomic sequences. 14
Table 2. TTCaV incidence in different regions in Republic of Korea. 17
Table 3. Comparison of ORF1, 2, and 3 of three strains with TTCaV reference strain (AB076002). 18
Table 4. Recombination region identified in MZ503912. 19
Figure 1. Phylogenetic tree generated using the partial sequences of TTCaV amplified by primer set Ⅰ and the reference strains. The 13... 20
Figure 2. Phylogenetic tree constructed using the complete genome of TTCaV and the reference TTV strain genomes. The maximum likelihood... 21
Figure 3. Similarity plot analysis using the three complete genomes of TTCaV isolated in this study and the Japanese reference strain, Cf-TTV10... 22
Figure 4. Recombination analysis using complete TTCaV genome. MZ503912 was considered a recombinant between MZ503910 strain... 23