Title Page
Contents
Abstract 7
1. Introduction 9
2. Materials and methods 11
2.1. Sample collection and RNA extraction 11
2.2. PSV diagnostic PCR 11
2.3. Virus isolation 11
2.4. Sequencing 12
2.5. Nucleotide and protein sequence analysis 12
2.6. Recombination detection 13
2.7. In-vitro cytokine induction 13
3. Result 15
3.1. Virus prevalence 15
3.2. Viral genome sequencing 17
3.3. Sequence Identity and Phylogenetic analysis 17
3.4. Recombination analysis 21
3.5. In-vitro cytokine induction 25
4. Discussion 27
5. Conclusion 30
References 34
Abstract (in Korean) 39
Table 1. Oligonucleotide primers used in this study to obtain nearly complete genome sequence 31
Table 2. Nucleotide and amino acid similarity of Polyprotein region(%) between PSV strain 20
Table 3. The p-values of seen methods of RDP for recombination analysis 22
Figure 1. Prevalence rates of PSV at pigs of different ages. 16
Figure 2. Phylogenetic tree based on polyprotein (a) and P1 region (b) nucleotide sequences of PSV strains. These trees were constructed by... 19
Figure 3. Recombination analysis between PSV strains. (A) Schematic view of recombination region between newly isolated PSV Korean strains. (B)... 24
Figure 4. The relative mRNA expression level of cytokines in PK-15 cells inoculated PSV in a time-course manner. 26