Title Page
Contents
ABSTRACT 9
Ⅰ. Introduction 11
1.1. Oats' usage as therapeutic plant 11
1.2. Skin barrier formation is critical for skin health 12
1.3. Purpose of the study 13
Ⅱ. Materials and Methods 14
2.1. Sample preparation 14
2.2. Mouse model of allergic contact dermatitis 14
2.3. Clinical evaluation and sample collection 15
2.4. Histopathological assessment 16
2.5. Cell culture and treatments 17
2.6. Real-time reverse transcription-polymerase chain reaction (qPCR) 17
2.7. Immunoblotting 21
2.8. Enzyme linked immunosorbent assay (ELISA) 23
2.9. Flow-cytometry 23
2.10. Statistical analysis 24
Ⅲ. Results 25
3.1. OSE modulates skin inflammatory reaction of the oxazolone-induced ACD model 25
3.2. OSE changes local cytokine secretion levels under the influence of the route of administration 31
3.3. OSE shows systemic immune- modulatory function via controlling T lymphocyte differentiation and proliferation in the oxazolone- induced ACD model 34
3.4. Epidermal barrier function was enhanced through modulation of structural protein contents after OSE treatment 37
3.5. OSE modulates different cell signaling pathways involved in skin proliferation and differentiation 40
3.6. Compounds of oat sprout mitigate inflammatory response in HaCaT irritation model 42
3.7. Compounds of oat sprout regulate epidermal barrier protein expression in HaCaT irritation model 44
Ⅳ. Discussions 46
Ⅴ. Conclusion 53
References 54
Abstract (in Korean) 59
Table 1. Scoring system for gross appearance of ACD mouse model. 16
Table 2. Primer sequences used in qPCR analysis. 19
Table 3. Antibodies used in immunoblotting. 22
Table 4. Antibodies used in flow-cytometry. 24
Figure 1. Macroscopic evaluation of the effect of OSE on oxazolone-induced ACD mouse model. (A) Changes in gross findings of skin lesions in oxazolone-sensitized... 27
Figure 2. Modulated systemic IgE and histamine concentration in the oxazolone-induced ACD mouse model with OSE treatment. Plasma IgE and histamine... 28
Figure 3. Microscopic evaluation of the effect of OSE on oxazolone-induced ACD mouse model. (A) Histological analysis of skin tissues from day 21 was performed.... 30
Figure 4. Immune mediator expression within local skin tissue in oxazolone-induced ACD mouse model with OSE treatment. (A) The expression of IL-1, IL-5, IL-6 and... 32
Figure 5. Flow cytometric analysis with splenocytes of oxazolone-induced ACD mouse model with OSE treatment. (A) A representative gating strategy for flow... 35
Figure 6. Skin moisturization effect of OSE on the oxazolone-induced ACD mouse model. (A) The effect of OSE treatment on the TEWL was measured on day 14 and... 38
Figure 7. Modulated EDC expression in the oxazolone-induced ACD mouse model with OSE treatment. (A) Expression of genes coding for barrier proteins from... 39
Figure 8. Modulation of cell signaling pathways in the oxazolone-induced ACD mouse model with OSE treatment. (A) Expression of signaling proteins in skin... 41
Figure 9. The effect of oat sprout compounds on cytokine expression in LPS-treated HaCaT. (A) Genetic expression of pro-inflammatory cytokines (IL-6, IL-8, TNFα,... 43
Figure 10. The effect of compounds of oat sprout on the expression of epidermal barrier proteins in LPS-treated HaCaT. The protein expression of profilaggrin,... 45