Title Page
Contents
ABSTRACT 8
Ⅰ. Introduction 10
Ⅱ. Materials and Methods 12
2.1. Cells and Viruses 12
2.2. RNA Extraction and Reverse Transcription 12
2.3. Construction of the rK148/08 Clone containing modified F Gene site 12
2.4. Virus Rescue and Propagation 13
2.5. Determination of Intracerebral pathogenicity index (ICPI) 14
2.6. Viral Growth Kinetics 14
2.7. Inactivated vaccine preparation 14
2.8. Vaccination and Challenge Experiments 15
2.9. Serological analysis 15
2.10. Statistical analysis 16
2.11. Ethics Statement 16
Ⅲ. Result 17
3.1. Construction of recombinant K148/08 vectors using reverse genetics system 17
3.2. Pathogenicity studies of recombinant viruses 17
3.3. Growth Properties of rK148/08 GRRQKR and K148/08 KJW 17
3.4. Immunogenicity evaluation of NDV inactivated vaccines in 6-week-old chickens 18
3.5. Protective efficacy of NDV inactivated vaccines in 6-week-old chickens 18
Ⅳ. Discussion 26
References 28
Abstract (in Korean) 30
Table 1. Intracerebral pathogenicity index and 50% egg infectious dose of viruses 20
Table 2. Overall scheme and fourth week results of the vaccination study with oil emulsified inactivated NDV vaccines 21
Table 3. Protective efficacy of vaccinated chickens and mock vaccinated chickens after the challenge with NDV Kr-005 22
Figure 1-1. Schematic representation of recombinant K148/08 NDV cDNA clone construction 23
Figure 1-2. Schematic description of the construction of recombinant pK148/08 GRRQKR 23
Figure 1-3. Schematic description of the construction of recombinant pK148/08 KJW 23
Figure 2. Viral growth kinetics of K148/08, K148/08 GRRQKR and K148/08 KJW in 10-day-old SPF embryonated chicken eggs 24
Figure 3. Serum antibody HI titer of vaccinated chickens for four weeks of vaccination against NDV LaSota antigen 25