Title Page
Contents
ABSTRACT 7
Ⅰ. INTRODUCTION 10
Ⅱ. MATERIALS AND METHODS 13
2.1. hiPSC culture 13
2.2. Confirmation of hiPSC pluripotency 13
2.3. HSCs manufacture and characterization 16
2.4. Expansion of HSCs 18
2.5. Differentiation of hiPSCs into erythroid cells 18
2.6. Confirmation of the differentiation of hiPSC-derived HSCs into erythroid cells 21
2.7. Analysis of surface marker expression by flow cytometry 23
Ⅲ. RESULTS 24
3.1. hiPSC culture and characterization of pluripotency 24
3.2. Generation and characterization of hiPSC-derived HSCs 27
3.3. Erythroid differentiation and characterization of hiPSCs 30
3.4. Differentiation of hiPSC-derived HSCs into erythroid cells and their characterization 33
3.5. Comparison of erythroid marker expression in control and experimental groups by CD34 expression 36
Ⅳ. DISCUSSION 39
Ⅴ. CONCLUSION 42
References 43
Abstract (in Korean) 50
Figure 1. hiPSC proliferation and tri-lineage differentiation period. 15
Figure 2. Production period of hiPSC-derived HSCs. 17
Figure 3. Direct differentiation period from hiPSC to erythroid cells. 20
Figure 4. Proliferation of hiPSC-derived HSCs and erythroid cell differentiation. 22
Figure 5. hiPSC morphology and lineage-specific marker expression. 26
Figure 6. HSC morphology and specific marker expression. 29
Figure 7. hiPSC-derived erythroid morphology and hematopoietic stem cell and erythroid cell surface marker expression. 32
Figure 8. Hematopoietic stem cells and erythroid cell marker expression in the experimental group. 35
Figure 9. Identification of erythroid-specific markers according to CD34 expression. 37