Title Page
Contents
ABSTRACT 9
1. Introduction 10
2. Materials and Methods 12
2.1. Cell culture 12
2.2. Isolation of EVs 12
2.3. Nanoparticle tracking analysis (NTA) 13
2.4. Western blot 13
2.5. Transmission electron microscopy (TEM) 14
2.6. Flow cytometry 14
2.7. qRT-PCR 15
2.8. Cell viability assay 17
2.9. Transwell migration assay 17
2.10. In vitro wound healing assay (scratch assay) 18
2.11. In vivo wound healing assay 18
2.12. Histological analysis 19
2.13. Statistical analysis 19
3. Results 20
3.1. Laminar flow-based bioreactor provides efficient production of EVs 20
3.2. CiR-EVs characterization 22
3.3. CiR-bioreactor regulates gene expression related to EV biogenesis. 26
3.4. CiR-EVs improved cell viability and migration in human fibroblasts 30
3.5. Wound healing effects of CiR-EVs in mouse skin wound model 33
4. Discussion 35
References 37
Abstract (in Korean) 42
Table 1. List of antibodies for western blot 14
Table 2. List of antibodies for flow cytometry 15
Table 3. List of primer for qRT-PCR 15
〈Figure 1〉 Schematic representation of of efficient production of EVs using CiR-bioreactor. 21
〈Figure 2〉 Characterization of CiR-EVs. 25
〈Figure 3〉 The effect of culture in CiR-Bioreactor on EV biogenesis. 29
〈Figure 4〉 In vitro wound healing effects of CiR-EVs. 32
〈Figure 5〉 In vivo wound healing effects of CiR-EVs. 34