표제지
목차
ABSTRACT 13
Ⅰ. 서론 16
1. 연구의 필요성 및 목적 16
2. 이론적 배경 18
2.1. 갱년기 여성의 피부노화 18
2.2. 식물성에스트로겐 20
2.3. 항산화 물질 22
2.4. 오미자 24
Ⅱ. 실험재료 및 방법 26
1. 실험재료 26
1.1. 재료 26
1.2. 시약 준비 26
1.3. 세포 준비 26
2. 실험방법 27
2.1. 오미자종자 추출물 제조방법 27
2.2. 오미자종자 추출물의 성분분석 기기 및 분석조건 28
2.3. 성분분석 표준용액의 조제 28
2.4. 세포 배양 29
2.5. 세포독성 측정 29
2.6. DPPH radical 소거 활성 측정 30
2.7. 콜라겐 및 히알루론산 합성효소 mRNA 발현량 측정 31
3. 오미자종자 추출물 화장품 제조 34
3.1. 오미자종자 추출물 화장품 제조방법 및 처방 34
3.2. 오미자종자 추출물 및 오미자종자 추출물 함유 화장품의 피부 안전성 확인 36
4. 오미자종자 추출물 함유 화장품의 인체적용시험 방법 39
4.1. 연구계획서 심의의뢰 39
4.2. 연구대상자 39
4.3. 시험부위 및 시험물질 적용 방법 42
5. 피부 측정 도구 및 방법 44
5.1. 시험장소 44
5.2. 안면홍조 평가 44
5.3. 항노화 평가 48
5.4. 미백 평가 52
5.5. 보습 평가 53
5.6. 재생 평가 56
6. 통계 분석 58
Ⅲ. 연구결과 및 고찰 59
1. 오미자종자 추출물의 schisandrin A, gomisin A, gomisin N 성분분석 결과 59
2. 오미자종자 추출물의 세포실험 결과 및 고찰 62
2.1. 세포독성 평가 결과 62
2.2. DPPH radical 소거활성 평가 결과 64
2.3. COL1A1 mRNA 발현량 평가 결과 66
2.4. HAS2 mRNA 발현량 평가 결과 68
3. 오미자종자 추출물 함유 화장품의 인체적용시험 결과 및 고찰 70
3.1. 연구대상자 정보 70
3.2. 연구대상자의 동질성 검증 70
3.3. 안면홍조 72
3.4. 항노화 83
3.5. 미백 105
3.6. 보습 109
3.7. 재생 119
Ⅳ. 결론 123
참고문헌 129
부록 150
[부록 1] 150
[부록 2] 154
[부록 3] 156
[부록 4] 157
[부록 5] 158
국문초록 159
〈Table 1-1〉 Conditions for HPLC analysis of schisandrin A, gomisin A and gomisin N 28
〈Table 1-2〉 Primer information used for gene expression analysis 33
〈Table 1-3〉 Composition table of cream-type cosmetics 35
〈Table 1-4〉 Judgment Criteria for International Contact Dermatitis Research Society 37
〈Table 1-5〉 Skin irritation test results for normal skin and sensitive skin 38
〈Table 1-6〉 Moisture-related face & body skin type (Corneometer) 40
〈Table 1-7〉 Applied samples for test sites by group per random classification 42
〈Table 2-1〉 Contents of schisandrin A, gomisin A and gomisin N in Schisandra chinensis seed extract 61
〈Table 2-2〉 Basic information of research subjects 70
〈Table 2-3〉 Homogeneity verification results of research subjects 71
〈Table 2-4〉 Facial skin erythema changes 73
〈Table 2-5〉 Heat-induced facial skin erythema changes 75
〈Table 2-6〉 Changes in facial skin temperature due to heat 80
〈Table 2-7〉 Facial skin elasticity changes 84
〈Table 2-8〉 Dermal density changes in facial skin 86
〈Table 2-9〉 Cheek lifting changes in facial skin 90
〈Table 2-10〉 Eye wrinkle changes in facial skin 94
〈Table 2-11〉 Facial skin tone changes 98
〈Table 2-12〉 Facial pigmentation changes 106
〈Table 2-13〉 Facial skin moisture changes 110
〈Table 2-14〉 Uf Value changes in facial skin 112
〈Table 2-15〉 TEWL Changes in the forearm area damaged by SLS 114
〈Table 2-16〉 Skin tone changes in the forearm area stained with DHA 120
〈Figure 1-1〉 Molecular structure of lignan. (National center for biotechnology information) 21
〈Figure 1-2〉 Classification of polyphenols. 23
〈Figure 1-3〉 Schisandra chinensis. (Pixabay) 25
〈Figure 2-1〉 Manufacturing process of Schisandra chinensis seed extract. 27
〈Figure 2-2〉 Microplate reader system. 29
〈Figure 2-3〉 International contact dermatitis research society judgment criteria. 38
〈Figure 2-4〉 Research design diagram. n umber of persons (N), control group (C), Schisandra chinesis seed group (S.C.S) 43
〈Figure 3-1〉 Spectrophotometer. 45
〈Figure 3-2〉 VISIA-CA. 45
〈Figure 3-3〉 Infrared irradiator. 46
〈Figure 3-4〉 JANUS-1. 46
〈Figure 3-5〉 Thermal Imager. 47
〈Figure 3-6〉 Ballistometer. 49
〈Figure 3-7〉 DUB-Skin Scanner. 49
〈Figure 3-8〉 ANTERA 3D. 51
〈Figure 3-9〉 SKIN-O-MAT. 54
〈Figure 3-10〉 Dermal Torque Meter. 54
〈Figure 3-11〉 Vapometer. 55
〈Figure 4-1〉 Standard curves for schisandrin A, gomisin A and gomisin N. 60
〈Figure 4-2〉 Chromatogram of schizndrin A, gomisin A and gomisin N components in Schisandra chinensis seed extract. 61
〈Figure 4-3〉 Effect of Schisandra chinensis seed extract on the viability in NHDFs. Data are presented as mean±SD of three... 63
〈Figure 4-4〉 DPPH radical scavenging activity of Schisandra chinensis seed extract. Data are presented as mean±SD of three... 65
〈Figure 4-5〉 Color changes in DPPH radical scavenging activity Schisandra chinensis seed extract. 65
〈Figure 4-6〉 Effect of Schisandra chinensis seed extract on COL1A1 mRNA expression in NHDFs. Data are presented as... 67
〈Figure 4-7〉 Effect of Schisandra chinensis seed extract on HAS2 mRNA expression in NHDFs. Data are presented as... 69
〈Figure 5-1〉 Improvement rate of facial skin erythema. Data are presented as mean±SD of three independent experiments.... 73
〈Figure 5-2〉 Improvement rate of facial skin erythema due to heat. Data are presented as mean±SD of three independent... 75
〈Figure 5-3〉 Heat-induced changes in facial skin erythema. control group (C), Schisandra chinesis seed group (S.C.S) 78
〈Figure 5-4〉 Improvement rate of increased facial skin temperature due to heat. Data are presented as mean±SD of three... 80
〈Figure 5-5〉 Improvement rate of facial skin elasticity. Data are presented as mean±SD of three independent experiments.... 84
〈Figure 5-6〉 Improvement rate of dermal density in facial skin. Data are presented as mean±SD of three independent... 86
〈Figure 5-7〉 Changes in dermal density in facial area. control group (C), Schisandra chinesis seed group (S.C.S) 88
〈Figure 5-8〉 Improvement rate of cheek lifting in facial area. Data are presented as mean±SD of three independent... 90
〈Figure 5-9〉 Changes in cheek lifting in facial area. control group (C), Schisandra chinesis seed group (S.C.S) 92
〈Figure 5-10〉 Improvement rate of eye wrinkles in facial area. Data are presented as mean±SD of three independent... 94
〈Figure 5-11〉 Changes in eye wrinkles in facial area. control group (C), Schisandra chinesis seed group (S.C.S) 96
〈Figure 5-12〉 Improvement rate of skin tone in facial area. Data are presented as mean±SD of three independent experiments.... 98
〈Figure 5-13〉 Changes in facial skin tone. control group (C), Schisandra chinesis seed group (S.C.S) 101
〈Figure 5-14〉 The anti-aging action mechanism of Schisandra chinensis seed extract. 104
〈Figure 5-15〉 Changes in facial pigmentation. control group (C), Schisandra chinesis seed group (S.C.S) 108
〈Figure 5-16〉 Improvement rate of facial skin moisture. Data are presented as the mean±SD of values excluding maxima and... 110
〈Figure 5-17〉 Improvement rate of Uf value in facial skin. Data are presented as mean±SD of three independent experiments.... 112
〈Figure 5-18〉 Improvement rate of TEWL in the forearm area damaged by SLS. Data are presented as the mean±SD of values... 114
〈Figure 5-19〉 Changes in TEWL in the forearm area damaged by SLS. control group (C), Schisandra chinesis seed group (S.C.S) 116
〈Figure 5-20〉 Skin tone changes in the forearm area stained with DHA. control group (C), Schisandra chinesis seed group (S.C.S) 122