표제지
목차
Abbreviation 11
국문초록 12
Ⅰ. 서론 14
Ⅱ. 실험재료 및 방법 20
1. 실험 재료 20
2. 실험 기구 및 시약 20
1) 실험 기구 20
2) 시약 22
3. 실험 방법 25
1) 생강나무 추출 및 활성 물질 분리 25
2) 생강나무 유래 afzelin의 근육 분화 활성 평가 31
3) 생강나무 유래 afzelin의 근원세포 노화 억제 활성 평가 32
4) 통계 처리 34
Ⅲ. 실험 결과 35
1. 생강나무의 활성 물질 분리 및 구조 결정 35
1) 화합물 1의 구조 36
2) 화합물 2의 구조 39
3) 화합물 3의 구조 41
4) 화합물 4의 구조 43
5) 화합물 5의 구조 45
2. 생강나무의 근원세포 분화 활성 47
1) 생강나무 유래 화합물의 근원세포 분화 활성 47
2) Afzelin의 근원세포 분화 활성 49
3) Afzelin의 myotube 손상 저해 활성 54
3. Afzelin의 근원세포 노화 억제 활성 57
1) Trypan blue assay 57
2) β-galactosidase 염색 57
3) Oil Red O 염색 59
Ⅳ. 고찰 61
Ⅴ. 결론 64
Ⅵ. 참고 문헌 65
ABSTRACT 73
Figure 1. Structures of flavonoids from Lindera obtusiloba. 15
Figure 2. Structures of lignans from Lindera obtusiloba. 16
Figure 3. Extraction and solvent fractionation of Lindera obtusiloba. 30
Figure 4. Structure of isolated compounds from Lindera obtusiloba. 35
Figure 5. ¹H-NMR spectrum (500 MHz, above) and ¹³C-NMR spectrum (125 MHz, below) of compound 1 (DMSO-d₆). 37
Figure 6. HMBC spectrum (500 MHz) of compound 1 (DMSO-d₆). 38
Figure 7. ¹H-NMR spectrum (500 MHz, above) and ¹³C-NMR spectrum (125 MHz, below) of compound 2 (DMSO-d₆). 40
Figure 8. ¹H-NMR spectrum (500 MHz, above) and ¹³C-NMR spectrum (125 MHz, below) of compound 3 (methanol-d₄). 42
Figure 9. ¹H-NMR spectrum (500 MHz, above) and ¹³C-NMR spectrum (125 MHz, below) of compound 4 (methanol-d₄). 44
Figure 10. ¹H-NMR spectrum (500 MHz, above) and ¹³C-NMR spectrum (125 MHz, below) of compound 5 (CDCl₃). 46
Figure 11. Effect of flavonoid compounds on MHC and Myogenin expression. 48
Figure 12. Dose-dependent myogenic activity of afzelin (1). 50
Figure 13. Dose-dependent myogenic activity of afzelin (2). 51
Figure 14. Day-dependent myogenic activity of afzelin. 53
Figure 15. Inhibitory effect of afzelin on dexamethasone-induced myotube atrophy. 55
Figure 16. Inhibitory effect of afzelin on MHC in dexamethasone-induced myotube atrophy. 56
Figure 17. Inhibitory effect of afzelin on C2-ceramide-induced C2C12 myoblast senescence. 58
Figure 18. Inhibitory effect of afzelin on lipid infiltration into C2C12. 60