표제지
목차
국문초록 14
Ⅰ. 서론 16
Ⅱ. 문헌 고찰 20
1. 아이스 플랜트 20
2. 드링크젤리 21
3. 연하 장애 23
Ⅲ. 재료 및 방법 26
1. 실험 재료 26
2. 아이스 플랜트의 부위별 성분 특성 분석 26
1) 일반성분 26
2) 총 식이섬유 27
3) 무기질 27
4) 아미노산 28
5) 피니톨 및 이노시톨 32
6) 페놀화합물 32
3. 아이스 플랜트의 부위별 이화학적 특성 분석 35
1) pH 35
2) 총 고형물 35
3) 염도 35
4) 색도 36
4. 아이스 플랜트의 부위별 항산화 특성 분석 36
1) 총 폴리페놀 함량 36
2) 총 플라보노이드 함량 37
3) DPPH 라디칼 소거 활성 37
4) ABTS⁺ 라디칼 소거 활성 38
5) Fe²⁺ 킬레이팅 활성 38
5. 아이스 플랜트의 부위별 전분 분해 저해 활성 특성 분석 39
1) α-glucosidase 저해 활성 39
2) α-amylase 저해 활성 40
6. 부위별 아이스 플랜트의 항염증 특성 분석 40
1) 세포 생존율 40
2) Nitric oxide 생성량 41
3) 염증성 사이토카인 활성 42
7. 아이스 플랜트 드링크젤리 적용 가능성 43
1) 아이스 플랜트 드링크젤리 제조 43
2) 이화학적 특성 47
3) 관능검사 48
4) 아이스 플랜트 드링크젤리의 최적화 49
8. 통계 분석 50
Ⅳ. 결과 및 고찰 51
1. 아이스 플랜트의 부위별 성분 특성 51
1) 일반성분 51
2) 총 식이섬유 51
3) 무기질 53
4) 아미노산 53
5) 피니톨 및 이노시톨 함량 59
2. 아이스 플랜트의 부위별 이화학적 특성 63
1) pH 63
2) 총 고형분 63
3) 염도 63
4) 색도 65
3. 아이스 플랜트의 부위별 항산화 특성 65
1) 총 폴리페놀 함량 65
2) 총 플라보노이드 함량 67
3) DPPH 자유 라디칼 소거 활성 69
4) ABTS⁺ 자유 라디칼 소거 활성 69
5) Fe²⁺ 킬레이팅 활성 72
6) 페놀화합물 72
4. 아이스 플랜트의 부위별 전분 분해 저해 활성 77
1) α-Glucosidase 저해 활성 77
2) α-amylase 저해 활성 79
5. 아이스 플랜트의 부위별 항염증 특성 80
1) 세포 생존율 80
2) Nitric oxide 생성량 83
3) 염증성 사이토카인 활성 86
6. 아이스 플랜트 드링크젤리 특성 95
1) 아이스 플랜트 드링크젤리의 IDDSI 기준적용 젤화제 농도 95
2) 최적 제조조건 확립 97
3) 최적점 도출 및 최적화된 아이스 플랜트 드링크젤리의 특성 108
4) 고령자 대상 수응도 평가 112
Ⅴ. 요약 및 결론 115
참고문헌 118
APPENDICES 13
APPENDIX 1. Standard curve of pinitol 137
APPENDIX 2. Standard curve of inositol 138
APPENDIX 3. Standard curve of total polyphenol contentby gallic acid 139
APPENDIX 4. Standard curve of total flavonoid content by Quercetin 140
APPENDIX 5. Sensory score sheet 141
ABSTRACT 147
Table 1. Instrumental parameters of ICP-OES for determining minerals 29
Table 2. Instrumental parameters of ICP-MS for determining minerals 30
Table 3. HPLC operating conditions to analysis for Ice plant amino acids 31
Table 4. HPLC operating conditins to analysis for Ice plant pinotol and inositol 33
Table 5. UHPLC operating conditions to analysis for Ice plant phenolic compounds 34
Table 6. Experimental design for drink jelly with Ice plant 44
Table 7. Proximate compositions of Ice plant by each part 52
Table 8. Mineral Contents of Ice plant by each part 54
Table 9. Amino acid contents of Ice plant by each part 55
Table 10. Pinitol and inositol contents of Ice plant by each part 60
Table 11. Physicochemical properties of Ice plant by each part 64
Table 12. Hunter color values of Ice plant by each part 66
Table 13. Total polyphenol and total flavonoid contents of Ice plant by each part 68
Table 14. DPPH free radical scavenging activities of Ice plant by each part 70
Table 15. ABTS⁺ free radical scavenging activities of Ice plant by each part 71
Table 16. Fe²⁺ chelating activity of Ice plant by each part 73
Table 17. Distribution of identified phenolic compounds in Ice plant by each part 74
Table 18. Inhibitory effects of α-glucosidase and α-amylase activity in Ice plant by each part 78
Table 19. Cell viability of Ice plant by each part in RAW 264.7 cells 81
Table 20. Nitric oxide production of Ice plant by each part 84
Table 21. Effects of Ice plant by each part on the TNF-α levels 88
Table 22. Effects of Ice plant by each part on the IL-6 levels 90
Table 23. Effects of Ice plant by each part on the IL-1β levels 93
Table 24. Physicochemical characteristics of drink jelly with Ice plant 98
Table 25. Predicted model equation for physicochemical properties of drink jelly with Ice plant 100
Table 26. Hunter Color values of drunk jelly with Ice plant 103
Table 27. Predicted model equation for hunter color values of drink jelly with Ice plant 105
Table 28. Sensory properties of drink jelly with Ice plant 107
Table 29. Predicted model equation for sensory properties of drink jelly with Ice plant 110
Table 30. Physicochemical properties of optimized drink jelly with Ice plant 113
Table 31. Sensory properties of optimized drink jelly with Ice plant 114
Figure 1. Manufacturing process flow of drink jelly with Ice plant 46
Figure 2. Chromatogram of amino acid in Ice plant cotyledon by HPLC 56
Figure 3. Chromatogram of amino acid in Ice plant stem by HPLC. 57
Figure 4. Chromatogram of amino acid in Ice plant leaf by HPLC. 58
Figure 5. Chromatogram of pinitol and inositol in Ice plant by each part. 61
Figure 6. Effect of Ice plant each part on the cell viability. 82
Figure 7. Effects of Ice plant each part on production of NO by LPS-stimulated Raw 264.7 cells. 85
Figure 8. Effects of Ice plant each part on production of TNF-α levels. 89
Figure 9. Effects of Ice plant each part on production of IL-6 levels. 91
Figure 10. Effects of Ice plant parts on production of the IL-1β levels. 94
Figure 11. Regression analysis of gum mix concentration for viscosity of drink jelly with Ice plant. 96
Figure 12. Perturbation plot and response surface plot of the effect of Apple juice(A) and Ice plant (B) on physicochemical... 99
Figure 13. Perturbation plot and response surface plot of the effect on Apple juice(A)and Ice plant(B) on color values of drink jelly... 104
Figure 14. Perturbation plot and response surface plot of the effect of Apple juice (A) and Ice plant (B) on sensory evaluation of drink jelly with Ice plant. 109
Figure 15. Perturbation plot and response surface plot of the effect of Apple juice (A) and Ice plant (B) on optimization of drink jelly with Ice plant 111