Title Page
Contents
국문초록 9
ABSTRACT 11
Ⅰ. Introduction 13
Ⅱ. Objectives 18
Ⅲ. Materials and Methods 19
3.1. Cells 19
3.2. Antibodies, chemicals, and reagents 19
3.3. Animals 19
3.4. ELISA 20
3.5. IHC (Immunohistochemistry) 20
3.6. Immunofluorescence (IF) 21
3.7. Statistical analysis 21
Ⅳ. Results 23
4.1. ELISA can be used as a detection method for PK analysis. 23
4.2. Administering Medications via Different Routes 25
4.3. The biodistribution of protein X can be detected via ELISA method. 27
4.4. Immunohistochemistry (IHC) staining is a technique used to detect and localize specific proteins or antigens. 29
4.5. Confocal fluorescence microscopy can be used to visualize better images. 31
4.6. Characterization of PK properties of Protein X in Serum 33
4.7. Targeting efficacy of both Protein X at different time points in Tumor tissue. 36
Ⅴ. Discussion 39
Ⅵ. Conclusion 42
Reference 43
Table 1. PK parameters value of active and passive protein X in serum 35
Table 2. PK parameters value of active and passive protein X in Tumor tissue 38
Figure 1. Describes the detection method for PK analysis 24
Figure 2. Route of administration of the protein X. 26
Figure 3. Biodistribution of protein X in different tissues. 28
Figure 4. Detection of tumor tissue targeting at different routes such as SC and IP by IHC. 30
Figure 5. Detection of tumor tissue targeting at different routes such as SC and IP by IF. 32
Figure 6. Active and Passive Protein X concentration in serum at different time points. 34
Figure 7. Protein X concentration in tumor at different time point. 37