Title Page
Contents
ABSTRACT 9
초록 11
Ⅰ. Introduction 13
Ⅱ. Materials and Methods 16
Ⅱ-1. Working principle 16
Ⅱ-2. Instrument setup 18
Ⅱ-3. Sample preparation 21
Ⅲ. Fabrication 23
Ⅳ. Results and Discussion 27
Ⅳ-1. Analytical evaluation using healthy blood spiked with cancer cell lines 27
Ⅳ-2. Clinical evaluation using blood of patients with prostate and pancreatic cancer 36
Ⅳ-2-1. Prostate cancer patients 37
Ⅳ-2-2. Pancreatic cancer patients 44
Ⅳ-3. Discussion 50
Ⅴ. Conclusions 52
Ⅵ. Reference 53
Table 1. Information of five healthy donor, a benign prostate hyperplasia (BPH) patient, and seventeen prostate cancer patients (to... 42
Table 2. Information of five pancreatic cancer patients. 49
Figure 1. The workflow of the CTC-dChip for isolating and identifying heterogeneous CTCs. (a) The sample preparation process involves using... 17
Figure 2. Photographs of the fabricated CTC-dChip, which consists of (a) a disposable microchannel and (b) a reusable magnetic gradient... 19
Figure 3. The CTC-dChip and its experimental set-up. The assembled CTC-dChip is placed on two layered permanent magnets. (i) After the... 20
Figure 4. Fabrication process of the CTC-dChip. (a) A 50 μm-height microchannel is patterned using SU-8 (3050, MicroChem Co.) on a glass... 25
Figure 5. Analyses of the efficacy of isolating CTCs using healthy blood spiked with pancreatic (PANC-1) or prostate (LNCaP) cancer cell lines.... 31
Figure 6. Comparison of the performance of the CTC-dChip and MACS using 2 mL of healthy blood spiked with 200 PANC-1 or LNCaP cells.... 33
Figure 7. Fluorescence-based live/dead assay for isolated LNCaP cells utilizing the CTC-dChip was performed with different antibody... 35
Figure 8. Photomicrographs of CTCs (marked by green arrows; →) and WBCs (indicated by red arrows; →), isolated from prostate cancer...[이미지참조] 39
Figure 9. Clinical evaluation with blood from prostate cancer patients. (a) The number of CTCs and (b) the contaminating WBCs isolated per... 41
Figure 10. Photomicrographs of CTCs (marked by green arrows; →) and WBCs (indicated by red arrows; →), isolated from pancreatic...[이미지참조] 46
Figure 11. Clinical evaluation with blood from pancreatic cancer patients. (a) The number of CTCs and (b) the contaminating WBCs... 48