표제지
목차
국문요약 8
1. Introduction 10
2. Materials and methods 14
2.1. Chemicals 14
2.2. Animals and ethics 14
2.3. Treatments 14
2.4. Tissue preparation 15
2.5. Sperm counting 15
2.6. Penis length evaluation 15
2.7. Paraffin tissue section 16
2.8. Enzyme-linked immunosorbent assay (ELISA) 16
2.9. Total RNA extraction 17
2.10. Reverse transcription-polymerase chain reactions (RT-PCR) 18
2.11. Statistical analysis 18
3. Results 22
4. Discussion 39
References 46
ABSTRACT 58
Table 1. Primer sequences used in this study. 20
Table 2. Effect of PTU and LT4 treatment on organ weight in rats. 31
Table 3. Effect of PTU and LT4 treatment on relative organ weight in rats. 32
Fig. 1. Locations of primer sequences used in this study. 21
Fig. 2. Effect of PTU and LT4 on body weight in rats. 29
Fig. 3. Changes in the histological structure of the thyroid gland and serum thyroxine levels in response to PTU and LT4 administration. 30
Fig. 4. Organ size comparison in rats treated with PTU and LT4. 33
Fig. 5. Effect of prepubertal propylthiouracil administration one the serum testosterone levels and expression of StAR and Hsd17b3 mRNA in the testis. 34
Fig. 6. Effect of PTU and LT4 on anogenital distance, penis length and diameter in rats. 35
Fig. 7. Representative photomicrographs of epididymis, prostate, preputial gland in the CON, PTU and P+LT4 groups. 36
Fig. 8. Representative photomicrographs of germ cells detached from the seminiferous tubules of the testis. 37
Fig. 9. Representative sperm counting photomicrographs and sperm counting evaluation in each group. 38