Title Page
Abstract
Contents
1. INTRODUCTION 10
2. METHODS AND MATERIALS 15
2.1. Drosophila stocks and husbandry 15
2.2. Immunofluorescence assay and microscopy 15
2.3. S2 Cell culture and transfection 17
2.4. Co-immunoprecipitation and Western blot analysis 17
3. RESULTS 19
3.1. A decrease in H3K4me3 levels in the thoc5 heterozygous background does not induce the thoc5 mutant phenotype 19
3.2. Increased H3K4me3 and H3K27ac levels in the thoc5 mutant background do not suppress the thoc5 mutant phenotype 20
3.3. Thoc5 interacts with Aly in an RNA-independent manner 21
3.4. Changes in the localization of tMAC proteins, Aly and Comr, in thoc5 mutant 22
5. DISCUSSION 31
REFERENCES 33
ABSTRACT IN KOREAN 38
Figure 1. The effect of knocking down trxG in the thoc5 heterozygous background 25
Figure 2. The effect of overexpressing trxG or knocking down PcG in the thoc5 mutant background 27
Figure 3. Western blot analysis of the interaction between Thoc5 and Aly by Co-IP 28
Figure 4. Analysis of localization of tMAC proteins, Aly and Comr, in thoc5 mutants 30