Title Page
Contents
LIST OF ABBREVIATIONS 8
GENERAL INTRODUCTION 12
REFERENCES 21
ABSTRACT 28
Ⅰ. INTRODUCTION 29
Ⅱ. MATERIAL AND METHODS 33
A. Plant samples 33
B. Chromosome spread preparation 33
C. Pre-labeled oligonucleotide probes (PLOPs) preparation 34
D. FISH 34
Ⅲ. RESULTS 40
A. Chromosome numbers, size, and morphology 40
B. The chromosomal positioning of rDNA and telomeric repeat sequences 40
Ⅳ. DISCUSSION 66
Ⅴ. CONCLUSION 70
Ⅵ. REFERENCES 71
초록 77
Table 1. List of eight Senna species and their published chromosome information. 36
Table 2. List of pre-labeled oligonucleotide probes utilized in this study. 39
Table 3. FISH analyses of the mitotic metaphase chromosomes of eight Senna species. 65
Fig. 1. The structure of plant chromosomes. 18
Fig. 2. A conceptual explanation of fluorescence in situ hybridization. 19
Fig. 3. A schematic diagram depicting the biological classifications of various repetitive DNA within the plant genome. 20
Fig. 4. The images of eight Senna species used in this study. 37
Fig. 5. A schematic diagram depicting the experimental procedure in Senna species. 38
Fig. 6. DAPI metaphase chromosome spreads of eight Senna species. 42
Fig. 7. Distribution of 5S rDNA on metaphase spreads of eight Senna species. 43
Fig. 8. Distribution of 45S rDNA on metaphase spreads of eight Senna species. 44
Fig. 9. Distribution of telomeric repeat on metaphase spreads of eight Senna species. 45
Fig. 10. FISH - 5S rDNA, 45S rDNA, and telomeric repeat metaphase spreads of eight Senna species. 46
Fig. 11. FISH metaphase spread of S. artemisioides. 47
Fig. 12. FISH metaphase spread of S. durangensis. 48
Fig. 13. FISH metaphase spread of S. pallida. 49
Fig. 14. FISH metaphase spread of S. reticulata. 50
Fig. 15. FISH metaphase spread of S. wislizeni. 51
Fig. 16. FISH metaphase spread of S. obtusifolia. 52
Fig. 17. FISH metaphase spread of S. tora. 53
Fig. 18. FISH metaphase spread of S. aphylla. 54
Fig. 19. FISH karyogram of S. artemisioides. 55
Fig. 20. FISH karyogram of S. durangensis. 56
Fig. 21. FISH karyogram of S. pallida. 57
Fig. 22. FISH karyogram of S. reticulata. 58
Fig. 23. FISH karyogram of S. wislizeni. 59
Fig. 24. FISH karyogram of S. obtusifolia. 60
Fig. 25. FISH karyogram of S. tora. 61
Fig. 26. FISH karyogram of S. aphylla. 62
Fig. 27. FISH karyogram of eight Senna species. 63
Fig. 28. FISH ideogram of eight Senna species. 64