The prevalence of attention deficit hyperactivity disorder(ADHD), which is particularly common in children and adolescents, has rapidly increased in the past five years. ADHD refers to hyperactivity, impulsivity, and difficulty maintaining attention. ADHD has a high heritability; the child of a woman with ADHD has a high possibility of inheriting it. Inherited ADHD does not end with short-term treatment, and it often requires therapeutic intervention for several years. Therefore, proper diet and treatment for pregnant women with ADHD is important in preventing ADHD in children. In this study, we aimed to verify the effects of plant-based omega-3 PUFA feeding on ADHD-related behaviors and indices in rat offsprings during gestation of ADHD dams. The hypertensive rats used in this study were reported to be well characterized as an ADHD model and meet the most validation criteria of ADHD, while Wistar-Kyoto rats were used as the control group. During gestation, ADHD dams were fed a plant-based omega-3 PUFA diet for 3 weeks. After birth, behavioral experiments(MBT, OFT, EPM, Y-maze, FST) were conducted to confirm ADHD-related symptoms in rat offspring, differences between the experimental groups were compared, and the organs were weighed. Furthermore, serum inflammatory cytokines(IL-1β, IL-6, TNF-α) and serotonin were detected using ELISA. In the hippocampus, the brain-derived neurotrophic factor(BDNF), its receptor TrkB, and the expression of AKT, ERK, and CREB expressed as sub-mechanisms were analyzed using Western blot. Results showed no difference in hyperactivity between groups. Interestingly, anxiety and impulsivity were significantly improved in the Omega-3 group, and memory and behavioral despair were alleviated. In addition, serum inflammatory cytokines decreased, and the expression of BDNF and TrKB in the hippocampus increased. Meanwhile, expression of AKT and CREB were significantly increased in the female treatment group, and no significant difference was observed in the body weight, tissue, and serum serotonin levels of offspring mice. Therefore, a plant-based omega-3 PUFA diet in pregnant women with ADHD can be an effective intervention to alleviate ADHD-induced symptoms through increased synthesis of BDNF.