Title Page
Contents
ABSTRACT 8
Ⅰ. Introduction 10
Ⅱ. Materials and Methods 13
2.1. Cell culture 13
2.2. Cell viability assay after skim milk treatment 13
2.3. Measuring Estrogen Receptor 1 and 2 gene Expression using Reverse Transcription followed by Quantitative PCR 14
2.3.1. RNA extraction 14
2.3.2. Reverse Transcription and Quantitative PCR 15
2.4. Measuring GABA AR1 Protein Expression through Immunoblotting 16
2.5. Statistical Analyses 17
Ⅲ. Results 18
3.1. Anti-proliferative effect of skim milk on MDA-MB-231 and MCF-7 cells 18
3.2. Effect of skim milk on the growth of prostate cancer cells (PC-3 and LNCaP-LN3) and normal adipose stem cells 20
3.3. Expression of estrogen receptor and neurotransmitter proteins after skim milk treatment 22
3.4. Growth and protein expression after treatment with the neurotransmitter GABA 24
Ⅳ. Discussion 26
Ⅴ. Conclusion 29
References 30
Abstract (in Korean) 34
〈Table 1〉 List of primers sequences for RT-PCR 16
〈Figure 1〉 Cell growth of breast cancer cells after skim milk treatment. 19
〈Figure 2〉 Cell growth of prostate cancer cells (PC-3 and LNCaP-LN3) and normal adipose stem cells after skim milk treatment. 21
〈Figure 3〉 Expression of signaling molecules (ESR 1, ESR 2, GABA AR1) in breast cancer cells after skim milk treatment. 23
〈Figure 4〉 Cell growth and GABA AR1 expression in breast cancer cells after GABA treatment. 25