Title Page
Contents
ABSTRACT 8
Ⅰ. Introduction 10
Ⅱ. Materials and Methods 13
2.1. Samples 13
2.2. Virus isolation and propagation 13
2.3. RNA Extraction and real-time RT-PCR 14
2.4. RT-PCR and S1 gene sequencing 14
Ⅲ. Results 15
3.1. Statistical analysis of real-time PCR and Hemagglutination test conducted on samples from 2020 to 2023 15
3.2. S1 sequencing analysis results of IBV isolated from samples from 2020 to 2023 16
Ⅳ. Discussion 30
References 33
Abstract (in Korean) 38
Table 1. Real-time RT-PCR primers and probe 17
Table 2. IBV S1 gene RT-PCR primers 18
Table 3. Real-time RT-PCR thermocycle information for detection of NDV and IBV 19
Table 4. RT-PCR thermocycle information for S1 gene 20
Table 5. Detailed analysis information of IBV isolated in this study 21
Figure 1. Detailed statistical data on IBV real-time PCR results for washing samples from 2020 to 2023 24
Figure 2. Detailed statistical data on IBV real-time PCR results for tissue samples from 2020 to 2023 25
Figure 3. Detailed statistical data on NDV real-time PCR results for washing samples from 2020 to 2023 26
Figure 4. Detailed statistical data on NDV real-time PCR results for tissue samples from 2020 to 2023 27
Figure 5. Detailed statistical data on NDV Hemagglutination test results for washing samples from 2020 to 2023 28
Figure 6. Detailed statistical data on Hemagglutination test results for tissue samples from 2020 to 2023 29