Title Page
Contents
ABSTRACT 8
Ⅰ. Introduction 10
Ⅱ. MATERIALS AND METHODS 14
2.1. Cell Culture and treatment 14
2.2. Experimental animals 15
2.3. FACS 15
2.4. RNA isolation and real-time quantitative PCR 16
2.5. Immunoblot analysis 18
2.6. Frozen section 19
2.7. Immunofluorescence analysis 19
2.8. Quantification of human interleukin-6 (IL-6) 20
2.9. Color Fundus Photography imaging 20
2.10. H&E staining 20
2.11. Statistical analysis 21
Ⅲ. RESULT 22
3.1. Effects of PTBP1 knockdown in RPE cells exposed to CC-HS 22
3.2. Regulation of inflammatory response in RPE cells with PTBP1 knockdown 25
3.3. Decreased p65 expression in RPE upon Ptbp1 knockdown in NaIO3-induced mouse model 27
3.4. Validation of other effects induced by PTBP1 knockdown in RPE 29
Ⅳ. DISCUSSION 33
References 35
Abstract (in Korean) 38
Table 1. Primer List (h: human, m: mouse) 17
Table 2. Antibody List 19
Fig. 1. Decreased Apoptosis in RPE cells with PTBP1 knockdown 25
Fig. 2. Reduced inflammatory response in RPE cells with PTBP1 knockdown 26
Fig. 3. RPE degeneration phenotype in mouse RPE with Ptbp1 knockdown 28
Fig. 4. No improvement in senescence in RPE cells with PTBP1 knockdown 29
Fig. 5. No neuronal conversion occurs in RPE cells with PTBP1 knockdown 30
Fig. 6. No changes in retina cells occur in mouse RPE with Ptbp1 knockdown 32