Title Page
Contents
ABSTRACT 7
Ⅰ. Introduction 8
Ⅱ. Material and methods 10
2.1. Reagents and chemicals 10
2.2. Animals 10
2.3. Hormone treatments 10
2.4. RNA extraction, RT-PCR, and real-time RT-PCR 11
2.5. Western blot analysis 11
2.6. Immunofluorescence staining 12
2.7. Reassessment of public datasets 13
2.8. Statistics 13
Ⅲ. Results 14
3.1. Regulation of NPM1 expression in ovariectomized mouse uterus by E₂ 14
3.2. Regulation of NPM1 expression in ovariectomized mouse uterus by estrogen receptor antagonist (ICI 182,780) 17
3.3. E₂ enhances the expression of ribosome biogenesis-related genes in the uterus of ovariectomized mice 20
3.4. E₂ enhances the expression of MYC target genes in the uterus of ovariectomized mice 25
3.5. Selection of ribosome biogenesis-related MYC target gene lists 28
3.6. Regulation of MYC expression in ovariectomized mouse uterus by E₂ 31
Ⅳ. Discussion 33
References[내용없음] 5
Abstract (in Korean) 36
〈Figure. 1〉 Regulation of NPM1 expression in ovariectomized mouse uterus by E₂. 16
〈Figure. 2〉 Regulation of NPM1 expression in ovariectomized mouse uterus by estrogen receptor antagonist (ICI 182,780). 19
〈Figure. 3〉 E₂ enhances the expression of ribosome biogenesis-related genes in the uterus of ovariectomized mice. 24
〈Figure. 4〉 GSEA results for Gene Ontology Biological Process (GOBP) and hallmark gene sets in ovariectomized mouse uteri with E₂ treatment. 27
〈Figure. 5〉 Selection of ribosome biogenesis-related MYC target gene lists. 30
〈Figure. 6〉 Regulation of MYC expression in ovariectomized mouse uterus by E₂. 32