Title Page
Contents
ABSTRACT 9
1. Introduction 10
2. Materials and Methods 13
2.1. Cell culture 13
2.2. Flow cytometry 13
2.3. quantitative RT-PCR 14
2.4. Colony-Forming unit assay 15
2.5. Cell growth kinetics 15
2.6. Cell cycle assay 15
2.7. Cell proliferation assay 16
2.8. DAPI staining assay 17
2.9. Generation of EVs-treated bFGF 17
2.10. EVs produced with WT-bFGF or TS-bFGF 18
2.11. Western blotting 18
2.12. In vitro migration assay 19
2.13. NO assay 19
2.14. Statistical analysis 20
3. Results 21
3.1. Characterization of WJ-MSCs exposed to either TS- bFGF or WT-bFGF 21
3.2. TS-bFGF increase cell proliferation 24
3.3. Characterization of Exosomes derived from WT-WJ- MSCs and TS-WJ-MSCs 27
3.4. TS-3D EVs demonstrated a notable increase in cell proliferation and wound closure compared to WT-3D EVs 30
3.5. WT-WJ-MSC and TS-WJ-MSC EVs' anti-inflammatory effects 33
4. Discussion 36
References 40
Abstract (in Korean) 45
〈Table 1〉 List of primer sequences used in qRT-PCR 14
〈Figure 1〉 Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) were characterized after treatment with WT-bFGF or TS-bFGF. 23
〈Figure 2〉 Proliferation of cells in TS-WJ-MSCs and WT-WJ-MSCs. 26
〈Figure 3〉 Characterization of WT-bFGF or TS-bFGF-treated WJ-MSC-derived exosomes. 29
〈Figure 4〉 Evaluation of EVs in wound healing and cell proliferation. 32
〈Figure 5〉 Evaluation of TS-3D and WT-3D EVs' anti-inflammatory properties. 35