Title Page
Contents
ABSTRACT 8
1. Introduction 10
2. Materials and methods 13
2.1. Sample collection and RNA extraction 13
2.2. PTV diagnostic PCR 13
2.3. Virus isolation 13
2.4. Sequencing 14
2.5. Nucleotide sequence analysis 15
2.6. In-vitro cytokine induction 15
3. Result 16
3.1. Phylogenetic analysis 16
3.2. In vitro interferon cytokine induction 18
3.3. In vitro pro-inflammatory cytokine induction 18
3.4. In vitro anti-inflammatory cytokine induction 22
4. Discussion 25
5. Conclusion 27
References 29
Abstract (in Korean) 35
Table 1. Oligonucleotide primers used in this study to obtain nearly complete genome sequence 28
Figure 1. Phylogenetic tree based on VP1 nucleotide sequences of PTV strains. These trees were constructed by maximum likelihood method using MEGA11 program. 17
Figure 2. The relative mRNA expression level of interferon cytokines in ST and PK-15 cells inoculated KU2022PTV01, 02 in a time-course manner. 19
Figure 3. The relative mRNA expression level of pro-inflammatory cytokines in ST and PK-15 cells inoculated KU2022PTV01 in a time-course manner. 20
Figure 4. The relative mRNA expression level of pro-inflammatory cytokines in ST and PK-15 cells inoculated KU2022PTV02 in a time-course manner. 21
Figure 5. The relative mRNA expression level of IL-10 cytokines in ST and PK- 15 cells inoculated KU2022PTV01 in a time-course manner. 23
Figure 6. The relative mRNA expression level of IL-10 cytokines in ST and PK- 15 cells inoculated KU2022PTV02 in a time-course manner. 24