Title Page
Contents
ABSTRACT 9
1. Introduction 10
2. Materials and Methods 12
2.1. Isolation and Culture of Porcine Adipose Derived-Stem Cells (ADSCs) and Wharton's Jelly MSCs (WJ-MSCs) 12
2.1.1. Piglet dorsal fat dissection adipose tissue(ADSCs) 12
2.1.2. Umbilical cord dissection and Wharton's jelly separation (WJ-MSCs) 12
2.1.3. Isolation and culture of mesenchymal stem cell from each tissue 12
2.2. Cell Proliferation Analysis 13
2.3. Cell Counting Assay Using Kit-8 (CCK-8) 13
2.4. Immunocytochemistry 14
2.5. Adipogenic, Chondrogenic, and Osteogenic Differentiation and Staining 14
2.6. Quantification of Oil red O 15
2.7. RNA Isolation and RT-qPCR 15
2.8. Oxygen Consumption Rate Analysis (OCR) 15
2.9. Glycolytic Rate Assay (ECAR) 16
2.10. Statistical Analysis 16
3. Results 18
3.1. Characterization of Porcine ADSCs and WJ-MSCs 18
3.2. Comparison of Proliferation Rate between ADSCs and WJ-MSCs 20
3.3. Adipogenic Differentiation Potential of ADSCs and WJ-MSCs 22
3.4. Chondrogenic and Osteogenic Differentiation Potential of ADSCs and WJ-MSCs 25
3.5. Comparative Analysis of Oxidative Phosphorylation in ADSCs and WJ-MSCs 28
3.6. Comparative Analysis of Glycolysis in ADSCs and WJ-MSCs 30
4. Discussion 32
References 37
Abstract (in Korean) 42
Table 1. Primers used for RT-qPCR. 17
Figure 1. Characterization of adipose-derived stem cells (ADSCs) and Wharton's Jelly-derived MSC(WJ-MSCs) 19
Figure 2. Proliferation assay of ADSCs and WJ-MSCs 21
Figure 3. Adipogenic differentiation and staining of ADSCs and WJ-MSCs and RT-qPCR analysis 24
Figure 4. Chondrogenic and Osteogenic differentiation and staining of ADSCs and WJ-MSCs and RT-qPCR analysis 27
Figure 5. Oxygen consumption rate (OCR) analysis of ADSCs and WJ-MSCs 29
Figure 6. Glycolytic function of ADSCs and WJ-MSCs in Early passage 31