Contents
DNA cleaving activity of 4'-bromoacetophenone-pyrrolecarboxamide / 전라옥 1
초록 1
[Preface] 1
Experimental 2
General procedures 2
DNA-cleaving experiment (Plasmid relaxation assay) 2
Preparation of the buffers 3
Preparation of the gels 3
Purification of calf thymus DNA 3
Preparation of 3'-32P(이미지참조) End Labeled 167 Base Pair Restriction Fragment 3
Cleavage and High-Resolution Gel Electrophoresis of restriction fragments 4
Footprinting Reaction 4
Results and Discussion 4
Incubation time dependence 5
Reaction time dependence 5
Cleavage of DNA by non-halogenated acetophenone 6
The correspondence of the DNA cleaving sites with the binding sites 7
Acknowledgements 8
References 8