Contents
Novel pharmacological activities of ethyl vanillin / 정현주 ; 박은희 1
Abstract 1
[Preface] 1
Materials and methods 2
1. Chemicals and fertilized eggs 2
2. Experimental animals 2
3. Cell culture 2
4. Chorioallantoic membrane (CAM) assay 2
5. Acetic acid-induced vascular permeability 3
6. Acetic acid-induced writhing response 3
7. Carrageenan-induced inflammation in the air pouch 3
8. Nitrite analysis 3
9. Western blotting 3
10. Determination of intracellular reactive oxygen species (ROS) 4
11. Determination of MMP-2 and -9 activities 4
12. MTT reduction assay 4
13. Statistical analysis 4
Results and discussion 4
1. Anti-angiogenic activity 4
2. In vivo evaluation of anti-inflammatory activity 5
3. Anti-nociceptive activity 6
4. Inhibitory effect on the NO production 6
5. Suppressive effect on the ROS level 7
6. Inhibition on the induction of metallopro-teinase-9 8
7. Cytotoxicity 8
Conclusions 8
Acknowledgements 9
References 9