In high speed railway bridges, dynamic analysis is important because of high passing velocity and moving load at the regular intervals, and damping ratio is a major parameter to predict dynamic responses. In this paper, damping ratios were estimated by using half power band width method and extended Kalman filter according to acceleration signal conditions, and a relationship between estimated damping ratios and representative values of bridge vibration was derived. From the results, damping ratios estimated from total ambient vibration were more reliable than only free vibration part. In case of using extended Kalman filter, the estimated damping ratios varying with RMQ(root mean quad), as one of representative values of bridge vibration, have more feasible trend. Thus, it is shown that further studies on reliabilities of estimated damping ratios are needed.