Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have
different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds
were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various
environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark
culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared
with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed
germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid
plantlets were successfully transferred to pot.