검정콩의 종피에 함유된 개별 안토시아닌의 축적양상을 밝
히고자 숙기가 다른 두 품종(일품검정콩, 흑청콩)을 파종기를
5월 15일, 5월 30일 그리고 6월 15일 3시기로 달리하여 립비
대성기(R6)인 개화기후 35일부터 5일 간격으로 시료를 채취하
여 개별 안토시아닌인 Cyanidin-3-Glucoside, Delphinidin-3-
Glucoside 및 Petunidin-3-Glucoside을 UPLC로 분석하여 얻
은 결과는 다음과 같다.
1. 종피에 안토시아닌이 축적되는 시기는 일품검정콩에서는
Cyanidin-3-Glucoside와 Delphinidin-3-Glucoside는 개화기후
35일에, Petunidin-3-Glucoside는 개화기후 50일경이었고, 흑청
콩에서는 Cyanidin-3-Glucoside는 개화기후 40일, Delphinidin-
3-Glucoside는 개화 후 55일, Pt3G는 개화기후 60일경이었다.
2. 품종별 종피내 안토시아닌 축적기간은 일품검정콩에서는
Delphinidin-3-Glucoside는 개화기후 35~70일, Cyanidin-3-
Glucoside는 개화기후 40~70일, Petunidin-3-Glucoside는 개화
기후 50~70일이 소요되었으며, 흑청콩에서는 Delphinidin-
3-Glucoside와 Cyanidin-3-Glucoside는 개화기후 40~80일,
Petunidin-3-Glucoside는 개화기후 60~80일이 소요되었다.
3. 안토시아닌 축적량은 품종에 관계없이 파종이 늦어질수
록 많았다.This experiment was conducted to evaluate accumulated pattern of individual anthocyanins
in the testa with black pigmentation of two soybean varieties(Ilpumgeomjeongkong and Heugcheongkong)
during grain filling under three different planting dates (May 15, May 30, and June 15).
Individual anthocyanin components of Cyanidin-3-Glucoside, Delphinidin-3-Glucoside, and Petunidin-
3-Glucoside were analyzed by UPLC. The samples for analysis were collected from two varieties at 5 day
intervals starting on the 35th day after flowering, when R6 starts. The Ilpumgeomjeongkong started to
accumulate Cyanidin-3-Glucoside and Delphinidin-3-Glucoside anthocyanins in the testa at the 35th day
after flowering. In case for Petunidin-3-Glucoside, it starts to accumulation around at the 50th day after
flowering. In case of Heugcheongkong, it showed to start accumulate around the 40th day for Cyanidin-
3-Glucoside, at 55th days for Delphinidin-3-Glucoside, and at 60th days for Petunidin-3-Glucoside after
flowering. In the accumulating period of anthocyanin components in the testa of two soybean varieties,
Delphinidin-3-Glucoside was around 35th to 70th days, and Cyanidin-3-Glucoside anthocyanins accumulated
from 40th to 70th days after flowering in Ilpumgeomjeongkong, whereas Delphinidin-3-Glucoside
and Cyanidin-3-Glucoside was from 40th to 80th days anthocyanins accumulated after Flowering in
Heugcheongkong. The later the sowing time for active accumulation of anthocyanins, the higher amount
of them regardless of the varieties.