Fusarium wilt, caused by three races of Fusarium oxysporum f. sp. lycopersici, is one of the most important disease of tomato (Solanum lycopersicum) worldwide. A total of 1,906 tomato accessions were screened for the resistance to Fusarium wilt using I-3 SNP marker and high resolution melting analysis. Results showed that 97 accessions were homozygous resistant, 8 accessions were heterozygous resistant and 1,801 were homozygous susceptible. Accessions containing resistance were identified in 65 accessions of S. lycopersicum var. lycopersicum, 13 accessions of S. lycopericum var. cerasiform, 8 accessions of S. pimpinellifolium, 3accessions of S. habrochaites, 3 accessions of S. corneliomulleri, 1 accession of S. galapagense, 3 accessions of S. peruvianum, 1 accession of S. chilense. For accurate evaluation of the Fusarium wilt resistance, however, screening to race 1 and race 2 and bio-assay still remain to be evaluated.