In this study, the optimal conditions for quercitrinextraction from Houttuynia cordata Thunb. (HC) wereevaluated using the response surface methodology (RSM).
The quercitrin was analyzed and method validated by ultraperformance liquid chromatography carried out with aphotodiode array detector (UPLC-PDA). Central compositedesign (CCD), a type of RSM, was applied to estimate theinfluence of three independent variables, the extractiontemperature, extraction time, and extraction concentration,on the quercitrin yield. Analysis of variance (ANOVA)showed a good model fit (r2=0.8863). The predictedoptimal quercitrin yield was 1.497% under an extractiontemperature of 80oC and an extraction time of 34 min in asolvent of 95% methanol. Using these optimal conditions,the experimentally obtained quercitrin yield was 1.483%(.03), which agrees closely with the predicted value.