This study aims to develop a product using e arthworm’s autolyzed peptides. After applying the earthworm conditioner before and after the chemical administration, this paper observed its treatment effect and retentivity. This article studied how the earthworm conditioner affects hair when it penetrates permed hair through basic physical property tests and SEM and observed its morphological changes. The experimental group treated with the conditioner having earthworm’s autolysate before and after the administration to permed hair had significantly thicker and heavier hair than the normal permed hair or the control group in general. Moreover, its retentivity according to the times of shampooing was significantly higher than the normal permed hair or the control group. Also, the experimental group showed a higher amino acid content than the normal hair or the control group. And the experimental group treated with the earthworm conditioner indicated higher wave efficiency of permed hair than the control group. The tensile force was also significantly higher in the experimental group than the normal hair or the control group. And this remained significantly high even after the 20 times of shampooing. In the observation with an optical microscope and S EM, the experiment al group had smooth surface with the surface of the outer layer neatly arranged. The arrangement of the scale remained the same even after the 10 times of shampooing. It is thought that the retentive force together with the scale arrangement was increased as the earthworm autolysate peptide filled in the matrix slipped out due to the damage and wrapped the surface by being adhered well because it has the strong polarization for the hair with the negative ion through the disufide (s-s) bond created in perming after the pretreatment of the chemical treatment and the electrostatic interaction created by the post-treatment. Putting these results together, it is thought that the earthworm autolysate peptide, a natural material, is valuable to apply as a new conditioner ingredient.