This study was intended to clarify pre-treatment effect of the earthworm autolysates of which molecular weight distribution are different
respectively in treating permanent wave on normal hair. We added the protease crude enzyme extract gotten by cultivating Bacillus
subtilis K-54 separated from cheonggukjang (bean-paste soup prepared with ground fermented soybeans) when making the earthworm
autolysates. In the meantime, we made the molecular weight distribution of earthworm autolysates became different through making the
additive amount of protease crude enzyme became different. We observed the molecular weight distribution of earthworm autolysates
using SDS-PAGE, and measured hair thickness, tensile strength, density analysis by methylene blue dyeing, wave formation and
maintenance strength with the protease crude enzyme (0.112 unit) after carrying out the pre-treatment on each autolysates. In the event of
making the earthworm autolysates, the highest rate for the permanent effect was shown at LbB50(0.112+0.0895?0 unit) of which
protease per 1 g earthworm was added by about 30 times. As the result of study, for securing quality as a pre-treatment agent in treating
permanent wave on normal hair, it was thought that it is more important to make the earthworm protein became the decompression
substances with the proper size than to make them became the low molecular protein.