To investigate the effect of intake of enzyme food which is known to help digestion and absorption of dietary nourishment on scalp hair of middle-aged women in 40's-60's, this study classified 30 middle-aged women into a control group(Cont.)(15) and enzyme food intake group(E)(15) and made E intake total 6g of enzyme food, 3 g with morning meal and 3 g with evening meal, for 12 weeks, then compared with the Cont.. As a result of measuring and comparing the number of hairs per unit area(2.8 mm×3.6 mm), the number of hairs per hair follicle, thickness, tensile strength, absorbance, surface chromaticity and amino acid content of scalp hair before and after the intake. It was resulted that the number of hairs, the number of hairs per hair follicle, thickness, tensile strength, and amino acid content decreased for Cont, and they increased by significant level for E. Absorbance, brightness, redness, yellowness of hairs increased for Cont., and significantly decreased for E. Therefore, it was identified that the intake of enzyme food makes positive effect in hair growth and condition of 40's-60's women with decreased digestive enzyme activity by aging and menopausal transition.