Contents
Carbamylated erythropoietin ameliorate the transforming growth factor beta-1-induced epithelial-to-mesenchymal transition / Hye-Myung Ryu ; Soon-youn Choi ; Yong-Lim Kim 1
ABSTRACT 1
Introduction 1
Methods 2
1. MDCK cell culture 2
2. EPOR and CD131 expression on MDCKs using flow cytometry analysis (FACS) 2
3. Immunocytochemistry 2
4. Immunoblotting 2
Results 5
1. EPOR and CD131 are expressed in MDCK cells 5
2. TGF-β1 stimulation increases expression of EMT markers in MDCK cells 5
3. CEPO and rhEPO treatment attenuated TGF-β-induced EMT in MDCK cells 5
4. CEPO and rhEPO treatment attenuated TGF-β1-induced up-regulation of Smad-2 phosphorylation in MDCK cells 5
Discussion 6
References 6