The knowledge-sharing activities of subsidiaries are expressed as knowledge reverse transfers, and the subsidiaries that contribute to the enhancement of the capabilities of multinational corporations in terms of knowledge are called centers of excellence (CoE). Moreover, we focus on active tendency as a proactive factor while prior studies have been conducted on knowledge accumulation and creation of subsidiaries as a passive role of subsidiaries. In this study, we focus on the entrepreneurial spirit of subsidiaries as the core of proactiveness. The attributes of subsidiaries are located at the bottom of the head office in the organizational structure and tend to be dependent on the head office. However, this study aims at statistically verifying the causal relationship between entrepreneurship and knowledge creation of subsidiaries. In addition, we examine the antecedents that may affect entrepreneurship to differentiate this study from previous studies. A total of 282 foreign companies with subsidiaries in Korea were selected as a sample and analyzed using the Structural Equation Method. Empirical results show that the degree of product autonomy and vision sharing of subsidiaries have a positive effect on entrepreneurship, and the effect of this has a positive effect on subsidiary company knowledge creation. On the other hand, market autonomy and influence of the subsidiaries’s position did not seem to have any effect on entrepreneurship.