Contents
Strain-specific detection of Kimchi starter Leuconostoc mesenteroides WiKim33 using multiplex PCR / Moeun Lee ; Jung Hee Song ; Ji Min Park ; Ji Yoon Chang 1
Abstract 1
I. Introduction 1
II. Material and Methods 2
1. Leu. mesenteroides WiKim33-specific primer design 2
2. DNA extraction and amplification by multiplex PCR 2
3. Examination of sensitivity and specificity 2
4. Preparation of kimchi 2
5. pH and acidity 2
6. Total viable bacteria, LAB counts, and WiKim33 population ratio in kimchi samples 2
III. Results and Discussion 3
1. Design of the multiplex primer set for Leu. mesenteroides WiKim33 detection 3
2. Detection limit of the designed primer set 4
3. Application of WiKim33-specific PCR for sensing starter-inoculated kimchi 5
IV. Summary and Conclusion 8
References 9