Contents
Characterization and species-specific detection of Vibrio parahaemolyticus-related species associated with mass mortality of ark shell (Scapharca broughtonii) / Ryunkyoung Oh ; Min Jeong Lee ; Nam-Sil Lee ; Young-Ok Kim ; Hee Jeong Kong ; Ju-Won Kim ; Jung Youn Park ; Bo-Hye Nam ; Dong-Gyun Kim 1
ABSTRACT 1
Introduction 1
Materials and methods 2
1. Isolation and identification of pathogenic bacterial strains 2
2. 8M 4-1 bacterial challenge test 3
3. Histology of infected ark shell tissues 3
4. Amplification of virulence genes 4
5. Development of a monitoring method 4
Results 5
1. Isolation, identification, and characterization of pathogenic bacterial strains 5
2. Experimental infection test 6
3. Histological analysis of infected ark shell tissues 6
4. Detection of virulence genes 7
5. Species-specific PCR detection assay for 8M 4-1 monitoring 7
Discussion 11
REFERENCES 13