Purpose: This study aimed to identify the effects of psychological need satisfaction in exercise and motivation for exercise on physical activity. Methods: Eighty-five women working in three retail and health service sectors completed self-reported questionnaires during September and November 2018. Data were analyzed using SPSS for Windows 24.0 through descriptive statistics, t-tests, and logistic regression analysis. Results: Logistic regression analysis showed that intrinsic motivation (OR=1.95, 95% CI: 1.10~3.47) and autonomy (OR=1.65, 95% CI: 1.00~2.70) affected female workers' physical activity. Conclusion: To improve physical activity among women working in retail and health service sectors, programs to improve autonomy and intrinsic motivation should be developed and examined.