Collagen is a natural, biocompatible, and biodegradable material with low immunogenicity, which is useful for tissue and organ formation and in multiple cellular functions. Moreover, Type I collagen is known to be a suitable material for medical applications.
In this study, acid-soluble Type I collagen isolated from fish skin was used to make nanofibers through electrospinning using a high concentration of collagen solutions prepared by dissolving the collagen to 18 wt% in 20 wt% acetic acid at 20 °C. The collagen and collagen nanofiber were Type I collagen subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The nonaqueous collagen nanofiber was heat-treated, which yielded a chemical crosslinking agent, such as polyacrylic acid, besides collagen.