Contents
Antimicrobial peptides encounter resistance of aureolysin during their action on Staphylococcus aureus biofilm / Subin Seo ; Jingyoung Jung ; Chang Yeop Kim ; Hyuncheol Kang ; In Hee Lee 1
Abstract 1
1. Introduction 1
2. Materials and Methods 2
2.1. Peptides and enzymes 2
2.2. Bacteria culture and biofilm formation 2
2.3. Crystal violet staining and colony counting 2
2.4. Anti-biofilm assays 2
2.5. High pressure liquid chromatography (HPLC), acid urea (AU)-PAGE, Western blotting analysis, and mass spectrometry 3
2.6. Statistical analysis 3
3. Results and Discussion 3
3.1. Biofilm formation 3
3.2. Anti-biofilm activity of Hgn 4
3.3. Hgn fragments (fHgns) in the supernatant of biofilm treated with Hgn 5
3.4. Aureolysin activity in the supernatant of biofilm treated with HG1 6
References 7