Contents
(The) novel membrane-type micro-system to assess the bonus effect of physiological and physical stimuli on bone regeneration / Yen-Ching Yang ; Qian-Hui Hong ; Kin Fong Lei ; Alvin Chao-Yu Chen 1
Abstract 1
1. Introduction 1
2. Materials and Methods 2
2.1. The Isolation of the Periosteal Cells 2
2.2. Fabrication of the Single-and Dual-Chamber Membrane-Type Micro-system 3
2.3. Measurement of Cell Orientation 4
2.4. Experimental Procedure 4
2.5. Investigation of mRNA Expression Using Real-Time Polymerase Chain Reaction (PCR) 4
2.6. Quantification of Cell Proliferation 4
2.7. Statistical analysis 5
3. Results and Discussion 5
3.1. Characterization of the Tensile Strain Generated by the Membrane 5
3.2. Differentiation of Rabbit Periosteal Cells Under Tensile Strain Stimuli 6
3.3. Differentiation of Periosteal Cells Under Co-culture of Different Cells 7
3.4. Differentiation of Periosteal Cells Under Co-culture of ADSC Cells and Different Tensile Strains 7
4. Conclusions 8
References 8