Collagen is used in various medical and cosmetic materials as a biopolymer. Inorder to use it for high-end medical fibers, its mechanical properties must be improved,and this can be achieved by conjugating it with fish-skin-based collagen and chitosan. Theconjugated collagen fibers can utilize the advantages of the materials and can be appliedto make wet-laid nonwovens for wound dressing. In this study, the conjugated collagenfibers were prepared by wet spinning and their surface, optical, and mechanical propertieswere analyzed based on the various compositions of collagen and chitosan. In addition,the wet-laid nonwovens were manufactured with bilayers, designed with an inner layer ofconjugated collagen fibers and an outer layer of cotton/chitosan spunlace nonwoven. Thecharacteristics of the wet-laid nonwovens in terms of their liquid-handling properties andthe distribution of their pore sizes were investigated. The results thus obtained show thatthe use of conjugated fibers in wet-laid nonwovens can affect their properties, which canbe controlled by adjusting the composition ratio.