Contents
DNA–gold nanoparticle conjugates for intracellular miRNA detection using surface-enhanced raman spectroscopy / Won-Jun Lee ; Kyeong-Jun Kim ; Md. Khaled Hossain ; Hyeon-Yeol Cho ; Jeong-Woo Choi 1
Abstract 1
1. Introduction 1
2. Materials and methods 2
2.1. Materials 2
2.2. Preparation of the mi-RANs 2
2.3. Confirmation of Nanoparticle's Conjugation 2
2.4. Cancer Cell Culture 3
2.5. Treatment of mi-RANs on the Breast Cancer Cells 3
2.6. Measurement of SERS on mi-RANs Treated Cells 3
2.7. Measurement of the miR200c Expression of the Breast Cancer Cells Through Reverse-Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) 3
2.8. Cytotoxicity Assay 3
3. Results and Discussion 3
3.1. Particles Conjugation and Characterization 3
3.2. Detection of miR-200c Expression Level in Breast Cancer Cells Through SERS Measurement 4
3.3. Detection of miR-200c Expression in Breast Cancer Cells Through RT-qPCR 5
3.4. Confirmation of miR-200c-Related Drug Resistance 6
4. Conclusion 6
References 7