Contents
SF-qPCR : strand displacement-based fast quantitative polymerase chain reaction / Jiae Kim ; Cheulhee Jung 1
Abstract 1
1. Introduction 1
2. Materials and Methods 3
2.1. Enzymes and Reagents 3
2.2. Three-Step qPCR Assay 3
2.3. Two-Step qPCR Assay 3
2.4. SARS-CoV-2 RNA Virus RT-qPCR Assay 3
3. Results and Discussion 3
3.1. Reducing a Three-Step qPCR to a Two-Step qPCR 3
3.2. Optimization of Annealing/Extension Temperature 3
3.3. Optimization of Time for Each Step 4
3.4. Optimization of the Denaturation Conditions 4
3.5. Comparison of SD Polymerase and Taq Polymerase 4
3.6. Diagnosis of SARS-CoV-2 RNA Virus via SF-qPCR 6
4. Conclusion 7
References 8