Contents
Customized multilayered tissue-on-a-chip (MToC) to simulate bacillus Calmette-Guérin (BCG) immunotherapy for bladder cancer treatment / Seungjin Lee ; Jung Hoon Kim ; Su Jeong Kang ; In Ho Chang ; Joong Yull Park 1
Abstract 1
1. Introduction 1
2. Materials and Methods 3
2.1. Fabrication of MToC 3
2.2. Cells and Reagents 3
2.3. Fabrication of Bio-printing Cell Block 3
2.4. MToC Assembly for Bio-printing Cell Block Cultivation 4
2.5. Simulation Model 6
2.6. Live/Dead Staining Assay 6
2.7. Cell Proliferation Assay 6
2.8. THP-1 Monocytes Migration Assay 6
2.9. Statistical Analysis 8
3. Results and Discussion 9
3.1. MToC Fabrication 9
3.2. Simulation of the Fluid Dynamics in MToC 9
3.3. Cell Viability in MToC 11
3.4. BCG-Induced THP-1. Migration in MToC 12
3.5. Limitations and Potential Applications 14
4. Conclusion 14
References 14