Contents 1
Separation of white blood cells in a wavy type microfluidic device using blood diluted in a hypertonic saline solution / Sanjay Mane ; Vadiraj Hemadri ; Siddhartha Tripathi 1
Abstract 1
List of symbols 1
1. Introduction 1
2. Tonicity Background 3
3. Methods and Materials 3
3.1. Microchannel Fabrication 3
3.2. Experimental Setup and Procedure 3
3.3. Cell Counting and Parameters of Interest 4
4. Working Principle of WBC Separation 4
5. Results and Discussion 5
5.1. Effect of Hypertonicity on Cell Diameter and Circularity 5
5.2. Effect of Hypertonicity on Hematocrit, Mass, and Volume of Cells 6
5.3. WBC Separation 7
5.4. Effect of Hematocrit 7
5.5. Effect of Flow Rate 9
5.6. Performance of the WBC Separation Channel 10
5.7. Viability and Activation Status of WBCs 11
6. Conclusions 12
Author Contributions 12
References 12