The purpose of this study was to investigate the relationship among Family-Friendly Organizational Culture, Work-Family Conflict and Burnout. For the purpose of study, 220 survey questionnaires were distributed among employees who are working for 5-Star Hotel in Busan area from 1st July, 2022 to 31th July, 2022. Due to the reliability of data, 17 samples were discarded and 203 samples were put to actual analysis. In order to accomplish the purpose of the study, the researcher conducted and analyzed by using SPSS Ver. 22.0 statistics package. The results of the study are as follows. First, Family-Friendly Organizational Culture which are consist of organizational time demands perceived career consequences managerial supports had significantly negative effects on work-family conflict and burnout. Second, Work-Family Conflict had significantly positive effects on burnout. This study shows that if a hotel company implements various family-friendly systems to maintain the work-life balance of its employees establishes a flexible working system so that employees can freely use it, and provides active support from supervisors and other organizations, Experience will reduce work-family conflict and burnout and increase work performance. For this, the introduction and implementation of the family-friendly system is important, but it is to form a consensus that the members of the organization are not affected by any negative effects in using the family-friendly system and to encourage them to actively use the system.