Contents 1
Highly efficient DNA reporter for CRISPR/Cas12a-based specific and sensitive biosensor / Seungjin Lee ; Deahan Nam ; Jung Soo Park ; Seokjoon Kim ; Eun Sung Lee ; Byung Seok Cha ; Ki Soo Park 1
Abstract 1
1. Introduction 1
2. Materials and Methods 2
2.1. Reagents and Materials 2
2.2. Preparation of crRNA 2
2.3. Cas12a-Catalyzed Trans-Cleavage Reaction with Various DNA Reporters 2
2.4. Bacterial Cell Culture and gDNA Isolation 3
2.5. Detection of Target gDNA Using CRISPR/Cas System in Combination with PCR 3
3. Results and Discussion 3
3.1. Optimization of the DNA Reporter 3
3.2. Detection of Target DNA Using CRISPR/Cas System Equipped with the New DNA Reporter 4
3.3. Detection of Target gDNA Using CRISPR/Cas System in Combination with PCR 5
4. Conclusion 6
References 7