Contents 1
Effect of periodical tensile stimulation on the human skin equivalents by magnetic stretching skin-on-a-chip (MSSC) / Kyunghee Kim ; Subin Jeong ; Gun Yong Sung 1
Abstract 1
1. Introduction 1
2. Results and Discussion 2
2.1. Application of Tensile Stimulation into the 3D Skin Equivalents 2
2.2. Surface Functionalization to Inhibit Contraction of Full-Thickness Human Skin Equivalents (HSEs) 2
2.3. Genes Expression by Tensile Stimulation Using Magnetic Stretching Chip Device 4
2.4. Stria Formation in 3D HSEs by Tensile Stimulation Using Magnetic Stretching Chip Device 7
2.5. Protein Expressions by Tensile Stimulation Using Magnetic Stretching Chip Device 8
2.6. Effect of Stimulation Mode 8
3. Conclusions 10
4. Materials and Methods 10
4.1. MSSC Production 10
4.2. Uniaxial Stretching of MSSC 11
4.3. Using the Gravity Flow System 11
4.4. Surface Functionalization 11
4.5. Cell Culture 11
4.6. Construction of a 3D Human Skin Equivalent Model 11
4.7. Measurement of Contraction 12
4.8. Histopathology and Immunohistochemistry Analysis 12
4.9. Real-Time Quantitative PCR 12
4.10. Statistical Analysis 12
References 13