Recently, 3D printing has been steadily growing in related technologies and markets because it has the advantage of making lighter and more complex products. Moreover, 3D printing of metallic materials has been commercialized with increasing number of applications in aerospace. This study presents the design of a 2U nanosatellite having an aluminum 3D printed structure and its structural analysis results at the launch environment. The volume and weight of the target model followed CubeSat's 2U standard, and the material used was AlSi10Mg. Based on this, static load analysis and modal analysis were performed on the structure using finite element analysis according to the launch environment standards required by GSFC-STD-7000. As a result of the analysis, it was confirmed that the 3D-printed frame is structurally safe in all cases, and it could sufficiently replace the general structural frame similar to the Al6000 and Al7000 series materials produced by the machining process.