Contents 1
Fabrication of cell spheroids for 3D cell culture and biomedical applications / Se Yeon Park ; Hye Jin Hong ; Hyun Jong Lee 1
Abstract 1
1. Introduction 1
2. Characteristics of Spheroids 2
2.1. Cell Types 2
2.2. Morphology of Spheroids 3
3. Fabrication Methods 4
3.1. Hanging Drop 5
3.2. Well Plate with Non-adhesive Surface 5
3.3. Agitation-Based Cell Culture 6
3.4. Micropatterned Substrate 6
3.5. Microfluidics 6
4. Biomedical Applications of Spheroids 7
4.1. Cell Therapy 7
4.2. Drug Screening/Cell-Based Assays 8
4.3. Tissue Engineering 10
4.4. Secretome-Based Therapy 12
4.5. Conclusions and Perspectives 13
References 15